期刊
NEUROBIOLOGY OF AGING
卷 34, 期 11, 页码 2465-2481出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2013.04.027
关键词
Butyrylcholinesterase; Astrocytes; Microglia; Cholinoceptive cells; Alzheimer's disease; BCHE genotype; Glial fibrillary acidic protein; S100B; Complement system; Cholinergic anti-inflammatory pathway
资金
- Swedish Medical Research Council [05817]
- Stockholm County Council
- Karolinska Institute
- Strategic Research Program in Neuroscience at Karolinska Institutet
- Karolinska Institute Foundations
- Loo & Hans Osterman Foundation
- Dementia Foundation (Demensfonden)
- Magnus Bergvalls Foundation
- Olle Engkvist Byggm stare Foundation
- Ake Wibergs Foundation
- Old Servants Foundation
- Swedish Alzheimer Foundation
- Brain Foundation
- Gun and Bertil Stohnes Foundation
- Ahlen Foundation
- Foundation for Ragnhild and Einar Lundstroms Memory [LSHB-CT-2005-512146]
- Swedish Brain Power
Butyrylcholinesterase (BuChE) activity is associated with activated astrocytes in Alzheimer's disease brain. The BuChE-K variant exhibits 30%-60% reduced acetylcholine (ACh) hydrolyzing capacity. Considering the increasing evidence of an immune-regulatory role of ACh, we investigated if genetic heterogeneity in BuChE affects cerebrospinal fluid (CSF) biomarkers of inflammation and cholinoceptive glial function. Alzheimer's disease patients (n = 179) were BCHE-K-genotyped. Proteomic and enzymatic analyses were performed on CSF and/or plasma. BuChE genotype was linked with differential CSF levels of glial fibrillary acidic protein, S100B, interleukin-1 beta, and tumor necrosis factor (TNF)-alpha. BCHE-K noncarriers displayed 100%-150% higher glial fibrillary acidic protein and 64%-110% higher S100B than BCHE-K carriers, who, in contrast, had 40%-80% higher interleukin-1b and 21%-27% higher TNF-alpha compared with noncarriers. A high level of CSF BuChE enzymatic phenotype also significantly correlated with higher CSF levels of astroglial markers and several factors of the innate complement system, but lower levels of proinflammatory cytokines. These individuals also displayed beneficial paraclinical and clinical findings, such as high cerebral glucose utilization, low beta-amyloid load, and less severe progression of clinical symptoms. In vitro analysis on human astrocytes confirmed the involvement of a regulated BuChE status in the astroglial responses to TNF-alpha and ACh. Histochemical analysis in a rat model of nerve injury-induced neuroinflammation, showed focal assembly of astroglial cells in proximity of BuChE-immunolabeled sites. In conclusion, these results suggest that BuChE enzymatic activity plays an important role in regulating intrinsic inflammation and activity of cholinoceptive glial cells and that this might be of clinical relevance. The dissociation between astroglial markers and inflammatory cytokines indicates that a proper activation and maintenance of astroglial function is a beneficial response, rather than a disease-driving mechanism. Further studies are needed to explore the therapeutic potential of manipulating BuChE activity or astroglial functional status. (C) 2013 Elsevier Inc. All rights reserved.
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