期刊
NEUROBIOLOGY OF AGING
卷 30, 期 7, 页码 1091-1098出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2007.10.011
关键词
Hypoxia; APP processing; beta- and gamma-cleavage; Macroautophagy
资金
- Science and Technology Commission of Shanghai Municipality [05DJ14007, 07DJ14005]
- Chinese National Sciences Foundation [30570560/C010601]
- 973 National project [2005CB724302]
Environmental factors are significant contributors for the development of Alzheimer's disease (AD). The greatly increased incidence of AD following stroke and cerebral ischemia suggests that hypoxia is a risk factor which may accelerate AD pathogenesis by altering amyloid precursor protein (APP) processing. However, the molecular mechanisms underlying the hypoxia mediated AD pathogenesis have not been fully elucidated. In the present study we demonstrated that repeated hypoxia increased beta-amyloid (A beta) generation and neuritic plaques formation by elevating beta-cleavage of APP in APP(swe) + PS1(A246E) transgenic mice. We also found that hypoxia enhanced the expression of APH-1a, a component of gamma-secretase complex, which in turn may lead to increase in gamma-cleavage activity. Furthermore, we demonstrated that repeated hypoxia treatment can activate macroautophagy, which may contribute to the increases in A beta production since pretreatment with macroautophagy inhibitor 3-methyladenine significantly blocked chemical hypoxic condition-induced increase in A beta production in SH-SY5Y cells. Taken together, our results suggest an important role of hypoxia in modulating the APP processing by facilitating both beta- and gamma-cleavage which may result in a significant increase of A beta generation. (C) 2007 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据