期刊
NEURO-ONCOLOGY
卷 17, 期 2, 页码 243-253出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/nou217
关键词
glioma; hedgehog; miRNA; SMO; stemness
资金
- National High Technology Research and Development Program 863 [2012AA02A508]
- China National Natural Scientific Fund [81372700]
- China Postdoctoral Science Foundation [2013M531121]
- Shanghai Postdoctoral Science Foundation [13R21411300]
- China Postdoctoral Science special Foundation [2014T70390]
Background. Few studies have associated microRNAs (miRNAs) with the hedgehog (Hh) pathway. Here, we investigated whether targeting smoothened (SMO) with miR-326 would affect glioma biological behavior and stemness. Methods. To investigate the expression of SMO and miR-326 in glioma specimens and cell lines, we utilized quantitative real-time (qRT)-PCR, Western blot, immunohistochemistry, and fluorescence in situ hybridization. The luciferase reporter assay was used to verify the relationship between SMO and miR-326. We performed cell counting kit-8, transwell, and flow cytometric assays using annexin-V labeling to detect changes after transfection with siRNA against SMO or miR-326. qRT-PCR assays, neurosphere formation, and immunofluorescence were utilized to detect the modification of self-renewal and stemness in U251tumor stem cells. A U251-implanted intracranial model was used to study the effect of miR-326 on tumor volume and SMO suppression efficacy. Results. SMO was upregulated in gliomas and was associated with tumor grade and survival period. SMO inhibition suppressed the biological behaviors of glioma cells. SMO expression was inversely correlated with miR-326 and was identified as a novel direct target of miR-326. miR-326 overexpression not only repressed SMO and downstream genes but also decreased the activity of the Hh pathway. Moreover, miR-326 overexpression decreased self-renewal and stemness and partially prompted differentiation in U251 tumor stem cells. In turn, the inhibition of Hh partially elevated miR-326 expression. Intracranial tumorigenicity induced by the transfection of miR-326 was reduced and was partially mediated by the decreased SMO expression. Conclusions. This work suggests a possible molecular mechanism of the miR-326/SMO axis, which can be a potential alternative therapeutic pathway for gliomas.
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