期刊
NEURO-ONCOLOGY
卷 13, 期 8, 页码 880-885出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/nor071
关键词
epidermal growth factor receptor; fluorescence in situ hybridization; fluorescence in situ gene protein assay; gene amplification; glioblastoma; proximity ligation assay
资金
- State of Illinois Excellence in Academic Medicine (EAM) [211]
- State of Alabama Investment Pool for Action (IMPACT)
We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein expression in cells and tissues. We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving gene and protein patterns simultaneously on a cell-by-cell basis.
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