期刊
NEURO-ONCOLOGY
卷 11, 期 6, 页码 757-766出版社
OXFORD UNIV PRESS INC
DOI: 10.1215/15228517-2009-014
关键词
B7-H1; brain; gliomas; tumor stem-like cells
资金
- National Natural Science Foundation of China [30772225, 30872655, 30700982]
- Program for Shanghai Outstanding Medical Academic Leaders [06027]
- Shanghai Municipality [07XD14005]
- Chinese national [20060400145]
Human glioblastoma is well known for its capacity to interfere with effective antitumor immune responses. B7-H1 is the third member of the B7 family that plays important roles in tumor immune evasion. Recent studies have shown that brain tumor stem-like cells (TSCs) contribute to tumorigenesis and radioresistance. However, the relationship between B7-H1 and the clinical behavior of brain TSCs remains unclear. In the present study, we report that B7-H1 is correlated with the malignancy grade of astrocyte tumors. B7-H1 was significantly upregulated at the growing edge of the tumors. Immunostaining and flow cytometric analysis indicate that B7-H1 was expressed primarily by Ki67-negative tumor cells. In vitro, tumors cultured under medium favoring the growth of neural stern cells were able to form spheres, along with expression of neural stem/progenitor cell markers. These cells were able to differentiate into different neural lineages when cultured in differentiation medium, indicating that these cells have TSC characteristics. We also found that B7-H1 was expressed, but not exclusively on CD133-positive stem cells. Interestingly, we found that CD133-negative tumor cells also had the capacity to form brain tumors. Our data establish a correlation between the expression of the negative costimulatory molecule B7-H1 and the malignancy grade of human gliomas, suggesting that B7-H1 may be a novel tumor marker and target for therapy, although it is not expressed exclusively on brain TSCs. Neuro-Oncology 11, 757-766, 2009 (Posted to Neuro-Oncology [serial online], Doc. D08-00272, March 5, 2009. URL http://neuro-oncology.dukejournals.org; DOI: 10.1215/15228517-2009-014)
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