期刊
NEURAL REGENERATION RESEARCH
卷 9, 期 5, 页码 540-548出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/1673-5374.130096
关键词
nerve regeneration; nerve root avulsion; spinal nerve root; heat shock protein 27; nitric oxide synthase; motor neurons; fluorescent antibody technique; choline acetyltransferase; a grant from Education Ministry of Jiangsu Province; Excellent Discipline of Jiangsu Province; neural regeneration
资金
- Education Ministry of Jiangsu Province [08KJB310002]
- Excellent Discipline of Jiangsu Province [JX10131801096]
In a previous study, heat shock protein 27 was persistently upregulated in ventral motor neurons following nerve root avulsion or crush. Here, we examined whether the upregulation of heat shock protein 27 would increase the survival rate of motor neurons. Rats were divided into two groups: an avulsion-only group (avulsion of the L-4 lumbar nerve root only) and a crush-avulsion group (the L-4 lumbar nerve root was crushed 1 week prior to the avulsion). Immunofluorescent staining revealed that the survival rate of motor neurons was significantly greater in the crush-avulsion group than in the avulsion-only group, and this difference remained for at least 5 weeks after avulsion. The higher neuronal survival rate may be explained by the upregulation of heat shock protein 27 expression in motor neurons in the crush-avulsion group. Furthermore, preconditioning crush greatly attenuated the expression of nitric oxide synthase in the motor neurons. Our findings indicate that the neuroprotective action of preconditioning crush is mediated through the upregulation of heat shock protein 27 expression and the attenuation of neuronal nitric oxide synthase upregulation following avulsion.
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