期刊
NEURAL DEVELOPMENT
卷 7, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1749-8104-7-26
关键词
-
资金
- Margaret E. Early Medical Research Trust
- Cedars-Sinai Regenerative Medicine Institute
- Department of Biomedical Sciences at Cedars-Sinai Medical Center
- National Institute of Health/National Institute on Neurologic Disorders and Stroke award [1R01NS076794-01]
- Alzheimer's Association Zenith Fellows Award [ZEN-10-174633]
- American Federation of Aging Research/Ellison Medical Foundation Julie Martin Mid-Career Award in Aging Research [M11472]
Background: Recent findings have indicated the presence of a progenitor domain at the marginal zone/layer 1 of the cerebral cortex, and it has been suggested that these progenitors have neurogenic and gliogenic potential. However, their contribution to the histogenesis of the cortex remains poorly understood due to difficulties associated with genetically manipulating these unique cells in a population-specific manner. Results: We have adapted the electroporation technique to target pial surface cells for rapid genetic manipulation at postnatal day 2. In vivo data show that most of these cells proliferate and progressively differentiate into both neuronal and glial subtypes. Furthermore, these cells localize to the superficial layers of the optic tectum and cerebral cortex prior to migration away from the surface. Conclusions: We provide a foundation upon which future studies can begin to elucidate the molecular controls governing neural progenitor fate, migration, differentiation, and contribution to cortical and tectal histogenesis. Furthermore, specific genetic targeting of such neural progenitor populations will likely be of future clinical interest.
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