期刊
NEPHROLOGY DIALYSIS TRANSPLANTATION
卷 28, 期 11, 页码 2754-2765出版社
OXFORD UNIV PRESS
DOI: 10.1093/ndt/gft278
关键词
mitochondrial DNA; mitochondrial K (K-ATP) channel; postconditioning; reactive oxygen species; renal protection
资金
- National Natural Science Foundation of China [30900591]
- Program of New Century Excellent Talents of Ministry of Education in China [NCET-10-0448]
Reactive oxygen species (ROS) play a major role in causing injury in ischemia-reperfusion (I/R). Mitochondrial DNA (mtDNA) is particularly vulnerable to oxidative damage. We propose that increased mitochondrial ROS production is likely to damage mtDNA, causing further injury to mitochondria, and postconditioning (POC) may ameliorate kidney I/R injury by mitigating mitochondrial damage. Rats were divided into seven groups: (i) Sham-operated animals with an unconstricted renal artery; (ii) Sham 5-hydroxydecanoate (5-HD); (iii) I/R; (iv) I/R 5-HD; (v) POC; (vi) Sham POC and (vii) POC 5-HD. Renal injury, oxidative DNA damage, mtDNA deletions, mitochondrial membrane potential (MMP) and expression of the ATP-sensitive K (K-ATP) channel subunit Kir6.2 were evaluated. Following 1 h of reperfusion, animals in the I/R group exhibited increased ROS, oxidative mtDNA damage shown by 8-hydroxy-2-deoxyguanosine staining, multiple base pair deletions and decreased MMP. However, POC rats exhibited less ROS, oxidative mtDNA damage and deletions and improved MMP. After 2 days of reperfusion, serum creatinine was elevated in I/R rats and the number of TdT-mediated dUTP nick-end labeled-positive tubular cells was increased and was associated with activation of caspase-3. Therefore, POC prevented the deleterious effects of I/R injury. Furthermore, the expression of mitochondrial Kir6.2 was widely distributed in renal tubular epithelial cells in Sham and POC rats and was lower in I/R rats. All of the protective effects of POC were reversed by the K (K-ATP) channel blocker 5-HD. POC may attenuate I/R injury by reducing mitochondrial oxidative stress and mtDNA damage and sustaining MMP.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据