期刊
NEONATOLOGY
卷 95, 期 2, 页码 117-124出版社
KARGER
DOI: 10.1159/000153095
关键词
Neonatal respiratory diseases; Respiratory distress syndrome; Surfactant proteins
类别
资金
- National Heart, Lung, and Blood Institute [HL065174, HL082747, HL065385, HL059959]
- Children's Discovery Institute of St. Louis Children's Hospital
- Saigh Foundation
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL059959, R01HL065174, R56HL059959, R01HL082747, R01HL065385] Funding Source: NIH RePORTER
Background: Genetic and developmental disruption of surfactant protein B (SP-B) expression causes neonatal respiratory distress syndrome (RDS). Objectives: To assess developmental and genetic regulation of SP-B expression in vivo. Methods: To evaluate in vivo developmental regulation of SP-B, we used immunoblotting to compare frequency of detection of mature and pro-SP-B peptides in developmentally distinct cohorts: 24 amniotic fluid samples, unfractionated tracheal aspirates from 101 infants >= 34 weeks' gestation with (75) and without (26) neonatal RDS, and 6 nonsmoking adults. To examine genetic regulation, we used univariate and logistic regression analyses to detect associations between common SP-B (SFTPB) genotypes and SP-B peptides in the neonatal RDS cohort. Results: We found pro-SP-B peptides in 24/24 amniotic fluid samples and in 100/101 tracheal aspirates from newborn infants but none in bronchoalveolar lavage from normal adults (0/6) (p < 0.001). We detected an association (p = 0.0011) between pro-SP-B peptides (M-r 40 and 42 kDa) and genotype of a nonsynonymous single nucleotide polymorphism at genomic position 1580 that regulates amino-terminus glycosylation. Conclusions: Pro-SP-B peptides are more common in developmentally less mature humans. Association of genotype at genomic position 1580 with pro-SP-B peptides (M-r 40 and 42 kDa) suggests genetic regulation of amino terminus glycosylation in vivo. Copyright (c) 2008 S. Karger AG, Basel
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据