期刊
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
卷 386, 期 6, 页码 541-550出版社
SPRINGER
DOI: 10.1007/s00210-013-0849-0
关键词
3-(2,2,2-Trimethylhydrazinium)-propionate; Carnitine palmitoyltransferase I; Mitochondria; Peroxisomes; PPAR alpha/PGC1 alpha pathway
资金
- Taiho Foundation
- ERDF [2010/0234/2DP/2.1.1.1.0/10/APIA/VIAA/063]
- L'Oreal Latvian fellowship
- European Social Fund [009/0147/1DP/1.1.2.1.2/09/IPIA/VIAA/009]
Depressed oxidation of long chain fatty acids (LCFA) in heart ischemia leads to acute accumulation of LCFA metabolites that impair the functioning of the mitochondria. We hypothesized that reduced activity of carnitine palmitoyltransferase-I (CPT-I) might activate peroxisomal LCFA oxidation and protect mitochondrial function in ischemia and reperfusion. In the present study, despite the long-term threefold reduction in L-carnitine content by 3-(2,2,2-trimethylhydrazinium)-propionate, the uptake and oxidation rates of LCFA in the heart in normoxia were not significantly influenced. The significant increase in PPAR alpha and PGC1 alpha nuclear content, observed in this study, were followed by increased expression of genes involved in peroxisomal fatty acid oxidation (FAO) which compensated for the limited CPT-I-dependent FA transport into the mitochondria. In ischemia followed by reperfusion, the redirection of LCFA oxidation from mitochondria to peroxisomes protected the mitochondria from the accumulation of LCFA. In turn, the recovery of FAO resulted in significant reduction of myocardial infarct size. In conclusion, the decreased L-carnitine content in the heart preserves its peroxisomal and mitochondrial function after ischemia and improves cardiac recovery during reperfusion. The functional interplay between the decrease in L-carnitine and the PPAR alpha/PGC1 alpha pathway-induced redirection of FA metabolism protects the mitochondria against LCFA overload and provides a foundation for novel cardioprotective mechanisms.
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