期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 20, 期 11, 页码 1250-U273出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.2679
关键词
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资金
- Howard Hughes Medical Institute
- University of Colorado Medical Scientist Training Program, US National Institutes of Health [T32 GM008497 (MSTP)]
- Fulbright Postdoctoral Fellowship
- Machiah Foundation Program
Polycomb repressive complex 2 (PRC2) is a histone methyltransferase required for epigenetic silencing during development and cancer. Long noncoding RNAs (lncRNAs) recruit PRC2 to chromatin, but the general role of RNA in maintaining repressed chromatin is unknown. Here we measure the binding constants of human PRC2 to various RNAs and find comparable affinity for human lncRNAs targeted by PRC2 as for irrelevant transcripts from ciliates and bacteria. PRC2 binding is size dependent, with lower affinity for shorter RNAs. In vivo, PRC2 predominantly occupies repressed genes; PRC2 is also associated with active genes, but most of those are not regulated by PRC2. These findings support a model in which PRC2's promiscuous binding to RNA transcripts allows it to scan for target genes that have escaped repression, thus leading to maintenance of the repressed state. Such RNAs may also provide a decoy for PRC2.
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