期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 19, 期 2, 页码 229-237出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.2204
关键词
-
资金
- Japan Science and Technology Agency
- Japan Society for the Promotion of Science
- Takeda Science Foundation
- Mochida Memorial Foundation for Medical and Pharmaceutical Research
- Cell Science Research Foundation
Core Q beta replicase comprise; the Q beta virus-encoded RNA-dependent RNA polymerase (beta-subunit) and the host Escherichia coli translational elongation factors EF-Tu and EF-Ts. The functions of the host proteins in the viral replicase are not clear. Structural analyses of RNA polymerization by core Q beta replicase reveal that at the initiation stage, the 3'-adenine of the template RNA provides a stable platform for de novo initiation. EF-Tu in Q beta replicase forms a template exit channel with the beta-subunit. At the elongation stages, the C-terminal region of the beta-subunit, assisted by EF-Tu, splits the temporarily double-stranded RNA between the template and nascent RNAs before translocation of the single-stranded template RNA into the exit channel. Therefore, EF-Tu in Q beta replicase modulates RNA elongation processes in a distinct manner from its established function in protein synthesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据