期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 18, 期 11, 页码 1211-U52出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.2149
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资金
- Canadian Institutes of Health Research (CIHR)
- Grants-in-Aid for Scientific Research [21229006] Funding Source: KAKEN
miRNAs recruit the miRNA-induced silencing complex (miRISC), which includes Argonaute and GW182 as core proteins. GW182 proteins effect translational repression and deadenylation of target mRNAs. However, the molecular mechanisms of GW182-mediated repression remain obscure. We show here that human GW182 independently interacts with the PAN2-PAN3 and CCR4-NOT deadenylase complexes. Interaction of GW182 with CCR4-NOT is mediated by two newly discovered phylogenetically conserved motifs. Although either motif is sufficient to bind CCR4-NOT, only one of them can promote processive deadenylation of target mRNAs. Thus, GW182 serves as both a platform that recruits deadenylases and as a deadenylase coactivator that facilitates the removal of the poly(A) tail by CCR4-NOT.
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