期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 17, 期 7, 页码 901-U175出版社
NATURE RESEARCH
DOI: 10.1038/nsmb.1829
关键词
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资金
- Max Planck Society
- German Research Foundation
- German-Israeli Foundation
- US National Institutes of Health
- Searle, Sandler and Keck Foundations
- International Human Frontier Science Program
- Israel Science Foundation
- Tel Aviv University
- Programa de Apoyo Sectorial a la Estrategia Nacional de Innovacion [DCI-ALA/2007/19.040]
- Agencia Nacional de Investigacion e Innovacion (ANII)
- Danish Council for Independent Research [DOK1155860]
- Lundbeckfonden [R45-A4342]
The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of similar to 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
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