期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 18, 期 1, 页码 27-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1934
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资金
- United States National Institutes of Health [GM 57071]
- Human Frontier Science Program
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM057071] Funding Source: NIH RePORTER
Expression of an individual gene can vary considerably among genetically identical cells because of stochastic fluctuations in transcription. However, proteins comprising essential complexes or pathways have similar abundances and lower variability. It is not known whether coordination in the expression of subunits of essential complexes occurs at the level of transcription, mRNA abundance or protein expression. To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells. Our results revealed that transcript levels of temporally induced genes are highly correlated in individual cells. In contrast, transcription of constitutive genes encoding essential subunits of complexes is not coordinated because of stochastic fluctuations. The coordination of these functional complexes therefore must occur post-transcriptionally, and likely post-translationally.
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