期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 17, 期 6, 页码 768-U147出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1807
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资金
- Royal Society [RG 2005/R1, BB/G011915/1]
- Marie Curie Actions
- European Union
- Biotechnology and Biological Sciences Research Council
- Cancer Research UK
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BB/G011915/1, BB/E013228/1] Funding Source: researchfish
- BBSRC [BB/E013228/1, BB/G011915/1] Funding Source: UKRI
Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall quality of the structure ensemble is good (backbone r.m.s. deviation of 0.48 angstrom) and agrees well with previously determined X-ray structures. Furthermore, measurements in more native-like small phospholipid bicelles indicate that the protein structure is the same as in detergent micelles, suggesting that environment-specific effects are minimal when using mild detergents. We use our case study as a platform to discuss the feasibility of similar solution NMR studies for other 7TM proteins, including members of the family of G protein-coupled receptors.
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