期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 16, 期 9, 页码 990-U117出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1659
关键词
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资金
- Israel Science Foundation [ISF 61/09, ISF 215/07]
- Joint Germany-Israeli Research Program [ca-139]
- Deutsche-Israel Project [DIP MI-1317]
- European Alternative Splicing Network (EURASNET)
- European Union [IRG-206872]
- Alon fellowship
An increasing body of evidence indicates that transcription and splicing are coupled, and it is accepted that chromatin organization regulates transcription. Little is known about the cross-talk between chromatin structure and exon-intron architecture. By analysis of genome-wide nucleosome-positioning data sets from humans, flies and worms, we found that exons show increased nucleosome-occupancy levels with respect to introns, a finding that we link to differential GC content and nucleosome-disfavoring elements between exons and introns. Analysis of genome-wide chromatin immunoprecipitation data in humans and mice revealed four specific post-translational histone modifications enriched in exons. Our findings indicate that previously described enrichment of H3K36me3 modifications in exons reflects a more fundamental phenomenon, namely increased nucleosome occupancy along exons. Our results suggest an RNA polymerase II-mediated cross-talk between chromatin structure and exon-intron architecture, implying that exon selection may be modulated by chromatin structure.
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