期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 16, 期 2, 页码 183-189出版社
NATURE RESEARCH
DOI: 10.1038/nsmb.1536
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资金
- American Cancer Society
- US National Institutes of Health [34431]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0745322] Funding Source: National Science Foundation
MicroRNAs (miRNAs) have been implicated in various cellular processes. They are thought to function primarily as inhibitors of gene activity by attenuating translation or promoting mRNA degradation. A typical miRNA gene produces a predominant similar to 21-nucleotide (nt) RNA (the miRNA) along with a less abundant amiRNA* product. We sought to identify miRNAs from the simple chordate Ciona intestinalis through comprehensive sequencing of small RNA libraries created from different developmental stages. Unexpectedly, half of the identified miRNA loci encode up to four distinct, stable small RNAs. The additional RNAs, miRNA-offset RNAs (moRs), are generated from sequences immediately adjacent to the predicted similar to 60-nt pre-miRNA. moRs seem to be produced by RNAse III-like processing, are similar to 20 nt long and, like miRNAs, are observed at specific developmental stages. We present evidence suggesting that the biogenesis of moRs results from an intrinsic property of the miRNA processing machinery in C. intestinalis.
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