期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 16, 期 12, 页码 1272-U90出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1689
关键词
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资金
- US National Science Foundation [0549593]
- National Institutes of Health (NIH) [GM58650, GM74252, GM74840, GM067153]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0549593] Funding Source: National Science Foundation
Transcription of eukaryotic genes by RNA polymerase II (Pol II) is typically accompanied by nucleosome survival and minimal exchange of histones H3 and H4. The mechanism of nucleosome survival and recovery of chromatin structure remains obscure. Here we show how transcription through chromatin by Pol II is uniquely coupled with nucleosome survival. Structural modeling and functional analysis of the intermediates of transcription through a nucleosome indicated that when Pol II approaches an area of strong DNA-histone interactions, a small intranucleosomal DNA loop (zero-size or (sic)-loop) containing transcribing enzyme is formed. During formation of the (sic)-loop, the recovery of DNA-histone interactions behind Pol II is tightly coupled with their disruption ahead of the enzyme. This coupling is a distinct feature of the Pol II-type mechanism that allows further transcription through the nucleosome, prevents nucleosome translocation and minimizes displacement of H3 and H4 histones from DNA during enzyme passage.
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