期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 15, 期 10, 页码 1119-1121出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1491
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20710170, 509, 521]
- New Energy and Industrial Technology Development Organization (NEDO)
- Grants-in-Aid for Scientific Research [20710170] Funding Source: KAKEN
Mitotic kinesin Eg5 is a homotetrameric molecular motor that cross-links and slides microtubules. The extent to which Eg5 moves processively is not clear. Here we use three-dimensional tracking of a quantum dot attached to the microtubule in a motility assay to directly visualize the corkscrew motion of a sliding microtubule. We show that the rotational pitch of microtubule sliding conveniently reports on the processivity of the driving motors, confirming that two-headed Eg5 is much less processive than two-headed kinesin-1.
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