期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 15, 期 6, 页码 598-604出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.1422
关键词
-
资金
- Medical Research Council [MC_U105184333] Funding Source: Medline
- Medical Research Council [MC_U105184333] Funding Source: researchfish
- MRC [MC_U105184333] Funding Source: UKRI
The telomeric G-overhangs of the ciliate Stylonychia lemnae fold into a G-quadruplex DNA structure in vivo. Telomeric G-quadruplex formation requires the presence of two telomere end binding proteins, TEBP alpha and TEBP beta, and is regulated in a cell-cycle dependent manner. Unfolding of this structure in S phase is dependent on the phosphorylation of TEBPb. Here we show that TEBP beta phosphorylation is necessary but not sufficient for a G-quadruplex unfolding rate compatible with telomere synthesis. The telomerase seems to be actively involved in telomeric G-quadruplex DNA structure unfolding in vivo. Significantly, the telomerase is recruited to telomeres by phosphorylated TEBP beta, and hence telomerase recruitment is cell-cycle regulated through phosphorylation. These observations allow us to propose a model for the regulation of G-quadruplex unfolding and telomere synthesis during the cell cycle.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据