期刊
NATURE REVIEWS GENETICS
卷 10, 期 10, 页码 669-680出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nrg2641
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资金
- National Institutes of Health [R01GM082798, U01HG004258, RL1DE019021]
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [U01HG004258] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [RL1DE019021] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM082798] Funding Source: NIH RePORTER
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a technique for genome-wide profiling of DNA-binding proteins, histone modifications or nucleosomes. Owing to the tremendous progress in next-generation sequencing technology, ChIP-seq offers higher resolution, less noise and greater coverage than its array-based predecessor ChIP-chip. With the decreasing cost of sequencing, ChIP-seq has become an indispensable tool for studying gene regulation and epigenetic mechanisms. In this Review, I describe the benefits and challenges in harnessing this technique with an emphasis on issues related to experimental design and data analysis. ChIP-seq experiments generate large quantities of data, and effective computational analysis will be crucial for uncovering biological mechanisms.
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