期刊
NATURE PROTOCOLS
卷 8, 期 6, 页码 1149-1154出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2013.055
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资金
- Novo Nordisk Foundation
- Swedish Cancer Foundation
- Swedish Research Council
- Torsten och Ragnar Soderbergs Stiftelser
- Peter Wallenberg Foundation for Economics and Technology
- Swedish Heart and Lung Foundation
- Ludwig Institute for Cancer Research
- Frans Wilhelm och Waldemar von Frenckells Fond
- Wilhelm och Else Stockmanns Stiftelse
- Karolinska Institutet
Excess lipid accumulation in peripheral tissues is a key feature of many metabolic diseases. Therefore, techniques for imaging and quantifying lipids in various tissues are important for understanding and evaluating the overall metabolic status of a research subject. Here we present a protocol that detects neutral lipids and lipid droplet (LD) morphology by oil red O (ORO) staining of sections from frozen tissues. The method allows for easy estimation of tissue lipid content and distribution using only basic laboratory and computer equipment. Furthermore, the procedure described here is well suited for the comparison of different metabolically challenged animal models. As an example, we include data on muscular and hepatic lipid accumulation in diet-induced and genetically induced diabetic mice. The experimental description presents details for optimal staining of lipids using ORO, including tissue collection, sectioning, staining, imaging and measurements of tissue lipids, in a time frame of less than 2 d.
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