期刊
NATURE PROTOCOLS
卷 8, 期 3, 页码 555-567出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2013.021
关键词
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资金
- US National Institutes of Health [R01 GM081617, R01 GM081563-04]
- New England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases [AI057159]
- Marie Curie Career Integration grant [303786]
- National Science Foundation [DMS-074876-0]
- Direct For Mathematical & Physical Scien
- Division Of Mathematical Sciences [0748760] Funding Source: National Science Foundation
We present a protocol for building and operating an automated fluidic system for continuous culture that we call the 'morbidostat'. The morbidostat is used to follow the evolution of microbial drug resistance in real time. Instead of exposing bacteria to predetermined drug environments, the morbidostat constantly measures the growth rates of evolving microbial populations and dynamically adjusts drug concentrations inside culture vials in order to maintain a constant drug-induced inhibition. The growth rate measurements are done using an optical detection system that is based on measuring the intensity of back-scattered light from bacterial cells suspended in the liquid culture. The morbidostat can additionally be used as a chemostat or a turbidostat. The whole system can be built from readily available components within 2-3 weeks by biologists with some electronics experience or engineers familiar with basic microbiology.
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