期刊
NATURE PROTOCOLS
卷 8, 期 9, 页码 1759-1764出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2013.085
关键词
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资金
- Engineering and Physical Sciences Research Council
- European Research Council starting investigator grant 'Naturale'
- Marie Curie Intra-European Fellowship within the 7th European Community Framework Programme
- Engineering and Physical Sciences Research Council [EP/E007627/1] Funding Source: researchfish
- EPSRC [EP/E007627/1] Funding Source: UKRI
This protocol describes a signal-generation mechanism for the naked-eye detection of analytes at low concentrations with ELISA. The key step is to generate solutions of desired tonality by growing gold nanoparticles with a particular state of aggregation. This is accomplished by linking the growth of gold nanoparticles with the biocatalytic cycle of the enzyme label. The protocol adapts a conventional ELISA procedure with catalase-labeled antibodies. The enzyme consumes hydrogen peroxide, and then gold (III) ions are added to generate gold nanoparticles. The concentration of hydrogen peroxide dictates the state of aggregation of gold nanoparticles. This allows for the naked-eye detection of analytes by observing the generation of blue- or red-colored gold nanoparticle solutions. When coupled with conventional ELISA, this signal-generation procedure allows for the naked-eye detection of analytes within 1 h.
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