期刊
NATURE PROTOCOLS
卷 7, 期 5, 页码 850-858出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2012.027
关键词
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资金
- Global Center of Excellence, Multidisciplinary Education and Research Center for the establishment of Regenerative Medicine (MERCREM)
- Innovation Center for Fusion of Advanced Technologies
- High-Tech Research Center from the Ministry of Education, Culture, Sports, Science and Technology (MEXST), Japan
- Japan Society for the Promotion of Science (JSPS)
The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.
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