4.7 Article

Integrated culture and purification of rat Schwann cells from freshly isolated adult tissue

期刊

NATURE PROTOCOLS
卷 7, 期 11, 页码 1996-2004

出版社

NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2012.118

关键词

-

资金

  1. Royal Thai Government Scholarship (Higher Educational Strategic Scholarships for Frontier Research Network, CHE-PhD SFR)
  2. BBSRC [BB/D524983/1] Funding Source: UKRI
  3. Biotechnology and Biological Sciences Research Council [BB/D524983/1] Funding Source: researchfish

向作者/读者索取更多资源

We describe a simple, rapid and highly selective protocol for the primary culture of Schwann cells in vitro from freshly dissociated adult rat nerve. The protocol is based on a selective culture medium comprising both mitogens (forskolin and optionally N2 supplement plus bovine pituitary extract), to stimulate growth of Schwann cells, plus an inhibitory substrate to simultaneously restrict fibroblast overgrowth (D-valine), contained in DMEM. This protocol differs from other available methods in that it uses the preferential capacity of Schwann cells to metabolize D-valine because of the difference in expression of a D-amino acid oxidase (DAAO) enzyme between Schwann cells and fibroblasts plus the presence of a selective mitogen to stimulate growth of Schwann cells. This permits derivation of highly pure Schwann cells directly from fresh adult nerve. Average Schwann cell purities of 97% can be achieved after 19 d without pre-degeneration, purification or antimitotic steps.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据