期刊
NATURE PROTOCOLS
卷 7, 期 8, 页码 1479-1496出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2012.075
关键词
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资金
- US National Institutes of Health [R01 HL094849, P20 HL101443, R01 HL084275, R01 HL107110, R01 HL110737, U54 HL081028]
- University of Miami Miller School of Medicine, Interdisciplinary Stem Cell Institute
Sustainable and reproducible large animal models that closely replicate the clinical sequelae of myocardial infarction (MI) are important for the translation of basic science research into bedside medicine. Swine are well accepted by the scientific community for cardiovascular research, and they represent an established animal model for preclinical trials for USUS Food and Drug Administration (FDA) approval of novel therapies. Here we present a protocol for using porcine models of MI created with a closed-chest coronary artery occlusion-reperfusion technique. This creates a model of MI encompassing the anteroapical, lateral and septal walls of the left ventricle. This model infarction can be easily adapted to suit individual study design and enables the investigation of a variety of possible interventions. This model is therefore a useful tool for translational research into the pathophysiology of ventricular remodeling and is an ideal testing platform for novel biological approaches targeting regenerative medicine. This model can be created in approximately 8-10 h.
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