期刊
NATURE PROTOCOLS
卷 5, 期 4, 页码 739-743出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2010.13
关键词
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资金
- Dutch Technology Foundation (STW) [VBC.6120, 10074]
- European Union
- ADAMANT
- CTMM, the Center for Translational Molecular Medicine [030-103]
- Direct For Mathematical & Physical Scien [1006711] Funding Source: National Science Foundation
The positron emitter zirconium-89 (Zr-89) has very attractive properties for positron emission tomography (PET) imaging of intact monoclonal antibodies (mAbs) using immuno-PET. This protocol describes the step-by-step procedure for the facile radiolabeling of mAbs or other proteins with Zr-89 using p-isothiocyanatobenzyl-desferrioxamine (Df-Bz-NCS). First, Df-Bz-NCS is coupled to the lysine-NH2 groups of a mAb at pH 9.0 (pre-modification), followed by purification using gel filtration. Next, the pre-modified mAb is labeled at room temperature by the addition of [Zr-89] Zr-oxalic acid solution followed by purification using gel filtration. The entire process of pre-modification, radiolabeling and purification steps will take about 2.5 h.
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