期刊
NATURE PROTOCOLS
卷 5, 期 2, 页码 201-208出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2009.222
关键词
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资金
- National Institutes of Health
- Ellison Medical Foundation/AFAR
- NATIONAL INSTITUTE ON AGING [R01AG027855] Funding Source: NIH RePORTER
Imaging neurons, glia and vasculature in the living brain has become an important experimental tool for understanding how the brain works. Here we describe in detail a protocol for imaging cortical structures at high optical resolution through a thinned-skull cranial window in live mice using two-photon laser scanning microscopy (TPLSM). surgery can be performed within 30-45 min and images can be acquired immediately thereafter. the procedure can be repeated multiple times allowing longitudinal imaging of the cortex over intervals ranging from days to years. Imaging through a thinned-skull cranial window avoids exposure of the meninges and the cortex, thus providing a minimally invasive approach for studying structural and functional changes of cells under normal and pathological conditions in the living brain.
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