期刊
NATURE PROTOCOLS
卷 4, 期 10, 页码 1454-1463出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2009.157
关键词
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资金
- Belgian FNRS/FRSM
- Belgian Queen Elizabeth Medical Foundation
- Simone et Pierre Clerdent Foundation
- Action de Recherches Concertees (ARC) Programs
- Interuniversity Attraction Poles Program (IUAP), Belgian State
- Federal Office
- Walloon Region Excellence Program CIBLES
- EU Marie Curie Fellowship Program
- FNRS
Embryonic stem cells (ESCs) constitute a tool of great potential in neurobiology, enabling the directed differentiation of specific neural cell types. We have shown recently that neurons of the cerebral cortex can be generated from mouse ESCs cultured in a chemically defined medium that contains no morphogen, but in the presence of the sonic hedgehog inhibitor cyclopamine. Corticogenesis from ESCs recapitulates the most important steps of cortical development, leading to the generation of multipotent cortical progenitors that sequentially produce cortical pyramidal neurons displaying distinct layer-specific identities. The protocol provides a most reductionist cellular model to tackle the complex mechanisms of cortical development and function, thereby opening new perspectives for the modeling of cortical diseases and the design of novel neurological treatments, while offering an alternative to animal use. In this protocol, we describe a method by which millions of cortical neurons can be generated in 2-3 weeks, starting from a single frozen vial of ESCs.
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