期刊
NATURE PROTOCOLS
卷 3, 期 10, 页码 1654-1659出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2008.149
关键词
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资金
- National Foundation for Scientific Research (FNRS)
- Universite catholique de Louvain (Fonds Speciaux de Recherche)
- Region wallonne
- Federal Office for Scientific, Technical and Cultural Affairs
- Research Department of the Communaute francaise de Belgique
Supported lipid bilayers (SLBs) are widely used in biophysical research to investigate the properties of biological membranes and offer exciting prospects in nanobiotechnology. Atomic force microscopy (AFM) has become a well-established technique for imaging SLBs at nanometer resolution. A unique feature of AFM is its ability to monitor dynamic processes, such as the interaction of bilayers with proteins and drugs. Here, we present protocols for preparing dioleoylphosphatidylcholine/dipalmitoylphosphatidylcholine (DOPC/DPPC) bilayers supported on mica using small unilamellar vesicles and for imaging their nanoscale interaction with the antibiotic azithromycin using AFM. The entire protocol can be completed in 10 h.
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