期刊
NATURE PROTOCOLS
卷 3, 期 10, 页码 1559-1568出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2008.147
关键词
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资金
- Institut National de la Sante et de la Recherche Medicale
- Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche (Neuroscience, Neurologie and Psychiatrie [06-Neuro-014-01]
- Fondation pour la Recherche Medicale
- Fondation Francaise pour la Recherche sur l'Epilepsie
- Ministry of Research
- ACI Jeunes Chercheurs
- European Community [LSHM-CT-2004-511995]
Analysis of synaptic transmission, synaptic plasticity, axonal processing, synaptic timing or electrical coupling requires the simultaneous recording of both the pre- and postsynaptic compartments. Paired-recording technique of monosynaptically connected neurons is also an appropriate technique to probe the function of small molecules (calcium buffers, peptides or small proteins) at presynaptic terminals that are too small to allow direct whole-cell patch-clamp recording. We describe here a protocol for obtaining, in acute and cultured slices, synaptically connected pairs of cortical and hippocampal neurons, with a reasonably high probability. The protocol includes four main stages (acute/cultured slice preparation, visualization, recording and analysis) and can be completed in similar to 4 h.
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