期刊
NATURE PHOTONICS
卷 8, 期 9, 页码 723-730出版社
NATURE PORTFOLIO
DOI: 10.1038/NPHOTON.2014.166
关键词
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资金
- National Cancer Institute of the US National Institutes of Health (NIH) [5R01CA135109-02]
- American Heart Association (Innovative Science Award)
- NIH [1R01NS064517]
- William S. Johnson Fellowship
To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography, with their limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-infrared regions (400-900 nm) is an alternative, but at present it requires craniotomy, cranial windows and skull-thinning techniques, and the penetration depth is limited to 1-2 mm due to light scattering. Here, we report through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy, utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3-1.4 mu m near-infrared window (NIR-Ila window). Reduced photon scattering in this spectral region allows fluorescence imaging to a depth of >2 mm in mouse brain with sub-10-mu m resolution. An imaging rate of similar to 5.3 frames per second allows for dynamic recording of blood perfusion in the cerebral vessels with sufficient temporal resolution, providing real-time assessment of a blood flow anomaly in a mouse middle cerebral artery occlusion stroke model.
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