期刊
SMALL
卷 11, 期 31, 页码 3797-3805出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201500251
关键词
cytotoxicity; heavy metal stress response; oxidative stress; silver nanoparticles
类别
资金
- US Public Health Service Grant through the NIEHS Centers for the Nanotechnology Health Implications Research (NCNHIR) Consortium [U19 ES019528]
- National Science Foundation
- Environmental Protection Agency [DBI 0830117, 1266377, RO1 ES016746]
While it is well known that there are interspecies differences in Ag sensitivity, differences in the cytotoxic responses of mammalian cells to silver nanoparticles (Ag NPs) are also observed. In order to explore these response outcomes, six cell lines, including epithelial cells (Caco-2, NHBE, RLE-6TN, and BEAS-2B) and macrophages (RAW 264.7 and THP-1) of human and rodent origin, are exposed to 20 nm citrate- and PVP-coated Ag NPs with Au cores, as well as 20 nm citrate-coated particles without cores. An MTS assay shows that while Caco-2 and NHBE cells are resistant to particles over a 0.1-50 g mL(-1) dose range, RAW 264.7, THP-1, RLE-6TN, and BEAS-2B cells are more susceptible. While there are small differences in dissolution rates, there are no major differences in the cytotoxic potential of the different particles. However, differences in anti-oxidant defense and metallothionein expression among different cell types are observed, which can partially explain differential Ag NP sensitivity. So, it is important to consider these differences in understanding the potential heterogeneous effects of nano Ag on mammalian biological systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据