期刊
NATURE NEUROSCIENCE
卷 16, 期 12, 页码 1754-1763出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nn.3563
关键词
-
资金
- Medical Research Council
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council
- German Research Foundation
- Brain Research Trust
- Special Trustees of the University College London Hospitals National Health Service Foundation Trust
- Epilepsy Research UK
- Worshipful Company of Pewterers
- European Research Council
- Action Medical Research [1725] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/H011900/1] Funding Source: researchfish
- Epilepsy Research UK [F0905] Funding Source: researchfish
- Medical Research Council [G0200373, MR/K000608/1, G0801316, G0600089, G116/147] Funding Source: researchfish
- BBSRC [BB/H011900/1] Funding Source: UKRI
- MRC [G0801316, G116/147, G0200373, MR/K000608/1, G0600089] Funding Source: UKRI
The role of voltage-gated Ca2+ channels (VGCCs) in spontaneous miniature neurotransmitter release is incompletely understood. We found that stochastic opening of P/Q-, N- and R-type VGCCs accounts for -50% of all spontaneous glutamate release at rat cultured hippocampal synapses, and that R-type channels have a far greater role in spontaneous than in action potentialevoked exocytosis. VGCC-dependent miniature neurotransmitter release (minis) showed similar sensitivity to presynaptic Ca2+ chelation as evoked release, arguing for direct triggering of spontaneous release by transient spatially localized Ca2+ domains. Experimentally constrained three-dimensional diffusion modeling of Ca2+ influx-exocytosis coupling was consistent with clustered distribution of VGCCs in the active zone of small hippocampal synapses and revealed that spontaneous VGCCs openings can account for the experimentally observed VGCC-dependent minis, although single channel openings triggered release with low probability. Uncorrelated stochastic VGCC opening is therefore a major trigger for spontaneous glutamate release, with differential roles for distinct channel subtypes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据