期刊
NATURE NEUROSCIENCE
卷 14, 期 4, 页码 513-U152出版社
NATURE PORTFOLIO
DOI: 10.1038/nn.2776
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资金
- Deutsche Forschungsgemeinschaft [Sonderforschungsbereich 807]
- Centre of Excellence Frankfurt Macromolecular Complexes
- Federal Ministry of Education and Research of Germany [01GQ0815]
- Max Planck Society
The light-gated cation channel channelrhodopsin-2 (ChR2) has rapidly become an important tool in neuroscience, and its use is being considered in therapeutic interventions. Although wild-type and known variant ChR2s are able to drive light-activated spike trains, their use in potential clinical applications is limited by either low light sensitivity or slow channel kinetics. We present a new variant, calcium translocating channelrhodopsin (CatCh), which mediates an accelerated response time and a voltage response that is similar to 70-fold more light sensitive than that of wild-type ChR2. CatCh's superior properties stem from its enhanced Ca2+ permeability. An increase in [Ca2+](i) elevates the internal surface potential, facilitating activation of voltage-gated Na+ channels and indirectly increasing light sensitivity. Repolarization following light-stimulation is markedly accelerated by Ca2+-dependent BK channel activation. Our results demonstrate a previously unknown principle: shifting permeability from monovalent to divalent cations to increase sensitivity without compromising fast kinetics of neuronal activation. This paves the way for clinical use of light-gated channels.
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