期刊
NATURE NEUROSCIENCE
卷 14, 期 4, 页码 527-U169出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nn.2765
关键词
-
资金
- The Robert Leet and Clara Guthrie Patterson Trust
- National Institute on Deafness and Other Communication Disorders (NIDCD) [K99]
- The Max-Planck Gesellschaft
- Medical Research Council (MRC)
- The Alexander Von Humboldt Foundation
- MRC [MC_U117597156] Funding Source: UKRI
- Medical Research Council [MC_U117597156] Funding Source: researchfish
Single-cell genetic manipulation is expected to substantially advance the field of systems neuroscience. However, existing gene delivery techniques do not allow researchers to electrophysiologically characterize cells and to thereby establish an experimental link between physiology and genetics for understanding neuronal function. In the mouse brain in vivo, we found that neurons remained intact after 'blind' whole-cell recording, that DNA vectors could be delivered through the patch-pipette during such recordings and that these vectors drove protein expression in recorded cells for at least 7 d. To illustrate the utility of this approach, we recorded visually evoked synaptic responses in primary visual cortical cells while delivering DNA plasmids that allowed retrograde, monosynaptic tracing of each neuron's presynaptic inputs. By providing a biophysical profile of a cell before its specific genetic perturbation, this combinatorial method captures the synaptic and anatomical receptive field of a neuron.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据