期刊
NATURE NANOTECHNOLOGY
卷 6, 期 10, 页码 668-674出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2011.147
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资金
- National Science Foundation [0546165]
- National Institutes of Health [GM079613]
- University of Missouri Intellectual Property Fast Track Initiative [A8881]
- Research Facilities Improvement Program
- National Centre for Research Resources, National Institutes of Health [C06-RR-016489-01]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0546165] Funding Source: National Science Foundation
MicroRNAs are short RNA molecules that regulate gene expression, and have been investigated as potential biomarkers because their expression levels are correlated with various diseases. However, detecting microRNAs in the bloodstream remains difficult because current methods are not sufficiently selective or sensitive. Here, we show that a nanopore sensor based on the alpha-haemolysin protein can selectively detect microRNAs at the single molecular level in plasma samples from lung cancer patients without the need for labels or amplification of the microRNA. The sensor, which uses a programmable oligonucleotide probe to generate a target-specific signature signal, can quantify subpicomolar levels of cancer-associated microRNAs and can distinguish single-nucleotide differences between microRNA family members. This approach is potentially useful for quantitative microRNA detection, the discovery of disease markers and non-invasive early diagnosis of cancer.
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