期刊
NATURE METHODS
卷 15, 期 10, 页码 793-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0139-3
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资金
- NIH National Institute of General Medical Sciences [F32GM128303, GM103310]
- Agency for Science, Technology and Research Singapore
- Simons Foundation [SF349247]
- NYSTAR
- Agouron Institute [F00316]
- NIH [OD019994]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM103310, F32GM128303] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [S10OD019994] Funding Source: NIH RePORTER
Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin.
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