期刊
NATURE METHODS
卷 15, 期 10, 页码 781-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0136-6
关键词
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资金
- European Research Council [648580, 680241]
- eScience Center [027016P04]
- NIH [1R21EB019589, P50GM085273]
- New Mexico Spatiotemporal Modeling Center
- International Max Planck Research School for Molecular and Cellular Life Sciences (IMPRS-LS)
- Max Planck Society
- Max Planck Foundation
- DFG [DFG JU 2957/1-1, SFB 1032]
- Center for Nanoscience (CeNS)
Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.
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