期刊
NATURE METHODS
卷 11, 期 7, 页码 723-U155出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2969
关键词
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资金
- US National Institutes of Health [5R01CA155320-04, P50 GM098792]
- Intelligent Synthetic Biology Center of Global Frontier Project - Ministry of Science, Information and Communication Technology and Future Planning of Korea [2013M3A6A8073557]
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
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