期刊
NATURE METHODS
卷 11, 期 9, 页码 927-930出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3043
关键词
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资金
- Howard Hughes Medical Institute
- Medical Research Council [U105184325]
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/F020384/1]
- Collaborative Computational Project [4 (CCP4)]
- BBSRC [BB/F020384/1] Funding Source: UKRI
- MRC [MC_U105184325] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F020384/1] Funding Source: researchfish
- Medical Research Council [MC_U105184325] Funding Source: researchfish
MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology.
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