期刊
NATURE METHODS
卷 11, 期 8, 页码 861-867出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2998
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资金
- US National Institute of General Medical Sciences [R01GM087227, R01GM087981]
- Norman Ackerman Advanced Research Program
- Robert A. Welch foundation [A-1769]
We report that a tetramethylrhodamine-labeled dimer of the cell-penetrating peptide TAT, dfTAT, penetrates live cells by escaping from endosomes with high efficiency. By mediating endosomal leakage, dfTAT also delivers proteins into cultured cells after a simple co-incubation procedure. We achieved cytosolic delivery in several cell lines and primary cells and observed that only a relatively small amount of material remained trapped inside endosomes. Delivery did not require a binding interaction between dfTAT and a protein, multiple molecules could be delivered simultaneously, and delivery could be repeated. dfTAT-mediated delivery did not noticeably affect cell viability, cell proliferation or gene expression. dfTAT-based intracellular delivery should be useful for cell-based assays, cellular imaging applications and the ex vivo manipulation of cells.
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