期刊
NATURE METHODS
卷 11, 期 10, 页码 1037-1040出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3068
关键词
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资金
- Howard Hughes Medical Institute
We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.
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