期刊
NATURE METHODS
卷 11, 期 12, 页码 1229-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3145
关键词
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资金
- US National Institutes of Health [EB00262, EB08396, EB001046, HL115553, GM065865, GM74048, GM57035]
- Howard Hughes Medical Institute
- US National Science Foundation
- American Heart Association
- Ruth L. Kirschstein National Research Service Award
- Novartis Foundation
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.
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