期刊
NATURE METHODS
卷 11, 期 6, 页码 625-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2925
关键词
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资金
- US National Institutes of Health (NIH) [R21 MH083614, R43 HD047089]
- NIH [DE16459]
- Center for Integrated Protein Sciences (EXC114)
- Munich Cluster for Systems Neurology (EXC1010)
- DFG [1373]
- Howard Hughes Medical Institute
sing a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NANA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mu m per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
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