期刊
NATURE METHODS
卷 11, 期 5, 页码 541-544出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2922
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资金
- UK Engineering and Physical Sciences Research Council
- RS Macdonald Charitable Trust
- Scottish Universities Life Sciences Alliance
- St Andrews 600 anniversary BRAINS appeal
- School of Biology
- Engineering and Physical Sciences Research Council [1287098, EP/J01771X/1] Funding Source: researchfish
- EPSRC [EP/J01771X/1] Funding Source: UKRI
Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian Light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.
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