期刊
NATURE METHODS
卷 11, 期 8, 页码 817-820出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3035
关键词
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资金
- UK Biotechnology and Biological Sciences Research Council [BB/J004499/1]
- UK Medical Research Council [MR/K011332/1]
- Wellcome Trust [0956451Z/11/Z]
- EU FP7 EpiGeneSys
- BLUEPRINT
- BBSRC [BBS/E/B/000C0400] Funding Source: UKRI
- MRC [G0801156, MR/K011332/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/B/000C0400] Funding Source: researchfish
- Medical Research Council [G0801156, MR/K011332/1] Funding Source: researchfish
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
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