期刊
NATURE METHODS
卷 10, 期 9, 页码 889-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2559
关键词
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资金
- Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- Japan Society for the Promotion of Science (JSPS)
- Strategic Research Program for Brain Sciences
- CREST-Japan Science and Technology Agency (JST)
- Strategic International Research Cooperative Program Japan-Mexico (SICPME-JST)
- Mitsubishi Foundation
- JSPS
- Grants-in-Aid for Scientific Research [25116507, 221S0003, 23680040, 25113705, 25560432, 25000015, 25115705] Funding Source: KAKEN
Identifying the neuronal ensembles that respond to specific stimuli and mapping their projection patterns in living animals are fundamental challenges in neuroscience. To this end, we engineered a synthetic promoter, the enhanced synaptic activity-responsive element (E-SARE), that drives neuronal activity-dependent gene expression more potently than other existing immediate-early gene promoters. Expression of a drug-inducible Cre recombinase downstream of E-SARE enabled imaging of neuronal populations that respond to monocular visual stimulation and tracking of their long-distance thalamocortical projections in living mice. Targeted cell-attached recordings and calcium imaging of neurons in sensory cortices revealed that E-SARE reporter expression correlates with sensory-evoked neuronal activity at the single-cell level and is highly specific to the type of stimuli presented to the animals. This activity-dependent promoter can expand the repertoire of genetic approaches for high-resolution anatomical and functional analysis of neural circuits.
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